A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus

A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was construc...

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Bibliografiske detaljer
Udgivet i:J Virol Methods
Main Authors: Nagesha, H.S, Wang, L.-F, Shiell, B, Beddome, G, White, J.R, Irving, R.A
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier Science B.V. 2001
Fag:
Brief Report
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7172176/
https://ncbi.nlm.nih.gov/pubmed/11164502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(00)00266-4
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