A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus

A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was construc...

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Bibliographic Details
Published in:J Virol Methods
Main Authors: Nagesha, H.S, Wang, L.-F, Shiell, B, Beddome, G, White, J.R, Irving, R.A
Format: Artigo
Language:Inglês
Published: Elsevier Science B.V. 2001
Subjects:
Brief Report
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7172176/
https://ncbi.nlm.nih.gov/pubmed/11164502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(00)00266-4
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