Biolistic transfection and expression analysis of acute cortical slices

BACKGROUND: Biolistic gene gun transfection has been used to transfect organotypic cultures (OTCs) or dissociated cultures in vitro. Here, we modified this technique to allow successful transfection of acute brain slices, followed by measurement of neuronal activity within a few hours. NEW METHOD: W...

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Bibliographic Details
Published in:J Neurosci Methods
Main Authors: Hamad, Mohammad I.K., Daoud, Solieman, Petrova, Petya, Rabaya, Obada, Jbara, Abdalrahim, Melliti, Nesrine, Stichmann, Sarah, Reiss, Gebhard, Herz, Joachim, Förster, Eckart
Format: Artigo
Language:Inglês
Published: 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7125026/
https://ncbi.nlm.nih.gov/pubmed/32119875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2020.108666
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