Biolistic transfection and expression analysis of acute cortical slices

BACKGROUND: Biolistic gene gun transfection has been used to transfect organotypic cultures (OTCs) or dissociated cultures in vitro. Here, we modified this technique to allow successful transfection of acute brain slices, followed by measurement of neuronal activity within a few hours. NEW METHOD: W...

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Detalhes bibliográficos
Publicado no:J Neurosci Methods
Main Authors: Hamad, Mohammad I.K., Daoud, Solieman, Petrova, Petya, Rabaya, Obada, Jbara, Abdalrahim, Melliti, Nesrine, Stichmann, Sarah, Reiss, Gebhard, Herz, Joachim, Förster, Eckart
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7125026/
https://ncbi.nlm.nih.gov/pubmed/32119875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2020.108666
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