Biolistic transfection and expression analysis of acute cortical slices

BACKGROUND: Biolistic gene gun transfection has been used to transfect organotypic cultures (OTCs) or dissociated cultures in vitro. Here, we modified this technique to allow successful transfection of acute brain slices, followed by measurement of neuronal activity within a few hours. NEW METHOD: W...

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Bibliografiske detaljer
Udgivet i:J Neurosci Methods
Main Authors: Hamad, Mohammad I.K., Daoud, Solieman, Petrova, Petya, Rabaya, Obada, Jbara, Abdalrahim, Melliti, Nesrine, Stichmann, Sarah, Reiss, Gebhard, Herz, Joachim, Förster, Eckart
Format: Artigo
Sprog:Inglês
Udgivet: 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7125026/
https://ncbi.nlm.nih.gov/pubmed/32119875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2020.108666
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