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Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
Biolistic transfection is a physical means of transfecting cells by bombarding tissue with high velocity DNA coated particles. We provide a detailed protocol for biolistic transfection of rat hippocampal slices, from the initial preparation of DNA coated bullets to the final shooting of the organoty...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2582847/ https://ncbi.nlm.nih.gov/pubmed/19066564 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/675 |
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