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Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome
We have developed a DNA assembly platform that utilizes the nonspecific, highly variable sequence signatures of type IIs restriction enzymes to assemble a full-length molecular clone of murine hepatitis coronavirus (MHV) strain A59. The approach also allows changes to be engineered into a DNA fragme...
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| Udgivet i: | SARS- and Other Coronaviruses |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7120124/ https://ncbi.nlm.nih.gov/pubmed/19057869 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-181-9_21 |
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