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Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59

A novel method was developed to assemble a full-length infectious cDNA of the group II coronavirus mouse hepatitis virus strain A59 (MHV-A59). Seven contiguous cDNA clones that spanned the 31.5-kb MHV genome were isolated. The ends of the cDNAs were engineered with unique junctions and assembled wit...

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Detalhes bibliográficos
Main Authors: Yount, Boyd, Denison, Mark R., Weiss, Susan R., Baric, Ralph S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC136593/
https://ncbi.nlm.nih.gov/pubmed/12368349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.76.21.11065-11078.2002
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