A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing

BACKGROUND: AsCas12a and LbCas12a nucleases are reported to be promising tools for genome engineering with protospacer adjacent motif (PAM) TTTV as the optimal. However, the C-containing PAM (CTTV, TCTV, TTCV, etc.) recognition by Cas12a might induce extra off-target edits at these non-canonical PAM...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Chen, Peng, Zhou, Jin, Wan, Yibin, Liu, Huan, Li, Yongzheng, Liu, Zhaoxin, Wang, Hongjian, Lei, Jun, Zhao, Kai, Zhang, Yiliang, Wang, Yan, Zhang, Xinghua, Yin, Lei
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7093978/
https://ncbi.nlm.nih.gov/pubmed/32213191
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-01989-2
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