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Programming PAM antennae for efficient CRISPR-Cas9 DNA editing
Bacterial CRISPR-Cas9 nucleases have been repurposed as powerful genome editing tools. Whereas engineering guide RNAs or Cas nucleases have proven to improve the efficiency of CRISPR editing, modulation of protospacer-adjacent motif (PAM), indispensable for CRISPR, has been less explored. Here, we d...
Enregistré dans:
| Publié dans: | Sci Adv |
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| Auteurs principaux: | , , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Association for the Advancement of Science
2020
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7209990/ https://ncbi.nlm.nih.gov/pubmed/32494703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aay9948 |
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