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A novel auto-cleavage assay for studying mutational effects on the active site of severe acute respiratory syndrome coronavirus 3C-like protease()
The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome (SARS) has been proposed as an attractive target for drug design. His(41) and Cys(145) were essential for the active site as the principal catalytic residues. In this study, we mutated the two sites, expressed four resulting mutant...
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| Yayımlandı: | Biochem Biophys Res Commun |
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| Asıl Yazarlar: | , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Elsevier Inc.
2004
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7092848/ https://ncbi.nlm.nih.gov/pubmed/15474466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2004.09.088 |
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