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A novel auto-cleavage assay for studying mutational effects on the active site of severe acute respiratory syndrome coronavirus 3C-like protease()
The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome (SARS) has been proposed as an attractive target for drug design. His(41) and Cys(145) were essential for the active site as the principal catalytic residues. In this study, we mutated the two sites, expressed four resulting mutant...
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| Publicado no: | Biochem Biophys Res Commun |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier Inc.
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7092848/ https://ncbi.nlm.nih.gov/pubmed/15474466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2004.09.088 |
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