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Precise large deletions by the PCR-based overlap extension method
The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparat...
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| Publicado no: | Mol Biotechnol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Humana Press
1995
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7090436/ https://ncbi.nlm.nih.gov/pubmed/8521036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/BF02907467 |
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