A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method

BACKGROUND: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. OBJECTIVES: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and gen...

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Veröffentlicht in:Iran J Biotechnol
Hauptverfasser: Karkhane, Ali Asghar, Yakhchali, Bagher, Rastgar Jazii, Ferdous, Bambai, Bijan, Aminzadeh, Saeed, Rahimi, Fatemeh
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Institute of Genetic Engineering and Biotechnology 2015
Schlagworte:
Brief Report
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5435007/
https://ncbi.nlm.nih.gov/pubmed/28959292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15171/ijb.1090
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