A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method

BACKGROUND: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. OBJECTIVES: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and gen...

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Библиографические подробности
Опубликовано в: :Iran J Biotechnol
Главные авторы: Karkhane, Ali Asghar, Yakhchali, Bagher, Rastgar Jazii, Ferdous, Bambai, Bijan, Aminzadeh, Saeed, Rahimi, Fatemeh
Формат: Artigo
Язык:Inglês
Опубликовано: National Institute of Genetic Engineering and Biotechnology 2015
Предметы:
Brief Report
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5435007/
https://ncbi.nlm.nih.gov/pubmed/28959292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15171/ijb.1090
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