A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method

BACKGROUND: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. OBJECTIVES: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and gen...

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Dettagli Bibliografici
Pubblicato in:Iran J Biotechnol
Autori principali: Karkhane, Ali Asghar, Yakhchali, Bagher, Rastgar Jazii, Ferdous, Bambai, Bijan, Aminzadeh, Saeed, Rahimi, Fatemeh
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Institute of Genetic Engineering and Biotechnology 2015
Soggetti:
Brief Report
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5435007/
https://ncbi.nlm.nih.gov/pubmed/28959292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15171/ijb.1090
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