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A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method
BACKGROUND: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. OBJECTIVES: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and gen...
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| Veröffentlicht in: | Iran J Biotechnol |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Institute of Genetic Engineering and Biotechnology
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5435007/ https://ncbi.nlm.nih.gov/pubmed/28959292 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15171/ijb.1090 |
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