A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method

BACKGROUND: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. OBJECTIVES: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and gen...

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Bibliografiske detaljer
Udgivet i:Iran J Biotechnol
Main Authors: Karkhane, Ali Asghar, Yakhchali, Bagher, Rastgar Jazii, Ferdous, Bambai, Bijan, Aminzadeh, Saeed, Rahimi, Fatemeh
Format: Artigo
Sprog:Inglês
Udgivet: National Institute of Genetic Engineering and Biotechnology 2015
Fag:
Brief Report
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5435007/
https://ncbi.nlm.nih.gov/pubmed/28959292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15171/ijb.1090
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