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A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments
BACKGROUND: Despite the ease of conventional splicing by overlap-extension (SOEing) PCR technique in theory, when splicing more than two fragments, and especially if one of the complementary sequences is A-T rich, the attachment of the fragments would be challenging. A new rapid and highly efficient...
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| Publicado no: | Avicenna J Med Biotechnol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Avicenna Research Institute
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7035467/ https://ncbi.nlm.nih.gov/pubmed/32153737 |
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