A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments

BACKGROUND: Despite the ease of conventional splicing by overlap-extension (SOEing) PCR technique in theory, when splicing more than two fragments, and especially if one of the complementary sequences is A-T rich, the attachment of the fragments would be challenging. A new rapid and highly efficient...

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Bibliografiske detaljer
Udgivet i:Avicenna J Med Biotechnol
Main Authors: Zarghampoor, Farzaneh, Behzad-Behbahani, Abbas, Azarpira, Negar, Khatami, Saeed Reza, Fanian, Maryam, Hossein Aghdaie, Mahdokht, Rafiei Dehbidi, Gholamreza
Format: Artigo
Sprog:Inglês
Udgivet: Avicenna Research Institute 2020
Fag:
Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7035467/
https://ncbi.nlm.nih.gov/pubmed/32153737
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