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Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

Zinc finger nuclease (ZFN) is a useful tool for endogenous site-directed genome modification. The development of an easier, less expensive and repeatedly usable construction method for various sequences of ZFNs should contribute to the further widespread use of this technology. Here, we establish a...

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Autori principali: Fujii, Wataru, Kano, Kiyoshi, Sugiura, Koji, Naito, Kunihiko
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3607563/
https://ncbi.nlm.nih.gov/pubmed/23536890
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0059801
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