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Generation of genetically modified mice using SpCas9-NG engineered nuclease
Although genetically modified mice can be generated with high efficiency by using CRISPR/Cas9-mediated genome editing in mouse zygotes, only the loci with a protospacer-adjacent motif (PAM) sequence are targetable. The present study investigated the usability of engineered Streptococcus pyogenes Cas...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6733909/ https://ncbi.nlm.nih.gov/pubmed/31501500 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49394-5 |
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