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A singular system with precise dosing and spatiotemporal control of CRISPR-Cas9

Several genome engineering applications of CRISPR-Cas9, an RNA-guided DNA endonuclease, require precision control of Cas9 activity over dosage, timing, and targeted site in an organism. While some control of Cas9 activity over dose and time have been achieved using small molecules, and spatial contr...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Manna, Debasish, Maji, Basudeb, Gangopadhyay, Soumyashree A., Cox, Kurt J., Zhou, Qingxuan, Law, Benjamin K., Mazitschek, Ralph, Choudhary, Amit
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7067309/
https://ncbi.nlm.nih.gov/pubmed/30834641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201900788
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