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Cas3/I-C mediated target DNA recognition and cleavage during CRISPR interference are independent of the composition and architecture of Cascade surveillance complex

In type I CRISPR-Cas system, Cas3—a nuclease cum helicase—in cooperation with Cascade surveillance complex cleaves the target DNA. Unlike the Cascade/I-E, which is composed of five subunits, the Cascade/I-C is made of only three subunits lacking the CRISPR RNA processing enzyme Cas6, whose role is a...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Nimkar, Siddharth, Anand, B
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7049708/
https://ncbi.nlm.nih.gov/pubmed/31980818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1218
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