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Heat-triggered remote control of CRISPR-dCas9 for tunable transcriptional modulation

CRISPR-associated proteins (Cas) are enabling powerful new approaches to control mammalian cell functions, yet the lack of spatially defined, noninvasive modalities limit their use as biological tools. Here, we integrate thermal gene switches with dCas9 complexes to confer remote control of gene act...

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Publicat a:ACS Chem Biol
Autors principals: Gamboa, Lena, Phung, Erick V., Li, Haoxin, Meyers, Jared P., Hart, Anna C., Miller, Ian C., Kwong, Gabriel A.
Format: Artigo
Idioma:Inglês
Publicat: 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7035993/
https://ncbi.nlm.nih.gov/pubmed/31904924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.9b01005
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