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Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 Å

HhaI, a Type II restriction endonuclease, recognizes the symmetric sequence 5′-GCG↓C-3′ in duplex DNA and cleaves (‘↓’) to produce fragments with 2-base, 3′-overhangs. We determined the structure of HhaI in complex with cognate DNA at an ultra-high atomic resolution of 1.0 Å. Most restriction enzyme...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Horton, John R, Yang, Jie, Zhang, Xing, Petronzio, Theresa, Fomenkov, Alexey, Wilson, Geoffrey G, Roberts, Richard J, Cheng, Xiaodong
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7026639/
https://ncbi.nlm.nih.gov/pubmed/31879785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1195
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