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Optimized Workflow for Multiplexed Phosphorylation Analysis of TMT-Labeled Peptides Using High-Field Asymmetric Waveform Ion Mobility Spectrometry

Phosphorylation is a post-translational modification with a vital role in cellular signaling. Isobaric labeling-based strategies, such as tandem mass tags (TMT), can measure the relative phosphorylation states of peptides in a multiplexed format. However, the low stoichiometry of protein phosphoryla...

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Bibliografske podrobnosti
izdano v:J Proteome Res
Main Authors: Schweppe, Devin K., Rusin, Scott F., Gygi, Steven P., Paulo, Joao A.
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6996458/
https://ncbi.nlm.nih.gov/pubmed/31799850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.9b00759
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