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Optimized Workflow for Multiplexed Phosphorylation Analysis of TMT-Labeled Peptides Using High-Field Asymmetric Waveform Ion Mobility Spectrometry

Phosphorylation is a post-translational modification with a vital role in cellular signaling. Isobaric labeling-based strategies, such as tandem mass tags (TMT), can measure the relative phosphorylation states of peptides in a multiplexed format. However, the low stoichiometry of protein phosphoryla...

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Detalhes bibliográficos
Publicado no:J Proteome Res
Main Authors: Schweppe, Devin K., Rusin, Scott F., Gygi, Steven P., Paulo, Joao A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6996458/
https://ncbi.nlm.nih.gov/pubmed/31799850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.9b00759
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