Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency

Versatile and precise genome modifications are needed for a wider range of adoptive cellular therapies(1–5). Here we report two improvements that increase the efficiency of CRISPR-Cas9-based genome editing in clinically relevant primary cell types. Truncated Cas9 target sequences (tCTS) added at the...

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Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Nguyen, David N., Roth, Theodore L., Li, P. Jonathan, Chen, Peixin Amy, Apathy, Ryan, Mamedov, Murad R., Vo, Linda T., Tobin, Victoria R., Goodman, Daniel, Shifrut, Eric, Bluestone, Jeffrey A., Puck, Jennifer M., Szoka, Francis C., Marson, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6954310/
https://ncbi.nlm.nih.gov/pubmed/31819258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-019-0325-6
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