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HPLC-Based Monitoring of Oxidation of Hydroethidine for the Detection of NADPH Oxidase-Derived Superoxide Radical Anion

Hydroethidine is a fluorogenic probe that in the presence of the superoxide radical anion is oxidized to a red fluorescent product, 2-hydroxyethidium. In cells, hydroethidine is also oxidized to other products, including red fluorescent ethidium. Thus, selective monitoring of 2-hydroxyethidium is re...

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Publicado en:Methods Mol Biol
Main Authors: Zielonka, Jacek, Zielonka, Monika, Kalyanaraman, Balaraman
Formato: Artigo
Idioma:Inglês
Publicado: 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6953631/
https://ncbi.nlm.nih.gov/pubmed/31172476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-9424-3_14
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