A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming

The Sleeping Beauty (SB) transposon is an advanced tool for genetic engineering and a useful model to investigate cut-and-paste DNA transposition in vertebrate cells. Here, we identify novel SB transposase mutants that display efficient and canonical excision but practically unmeasurable genomic re-...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Kesselring, Lisa, Miskey, Csaba, Zuliani, Cecilia, Querques, Irma, Kapitonov, Vladimir, Laukó, Andrea, Fehér, Anita, Palazzo, Antonio, Diem, Tanja, Lustig, Janna, Sebe, Attila, Wang, Yongming, Dinnyés, András, Izsvák, Zsuzsanna, Barabas, Orsolya, Ivics, Zoltán
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2020
Témata:
Molecular Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6943129/
https://ncbi.nlm.nih.gov/pubmed/31777924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1119
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