A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming

The Sleeping Beauty (SB) transposon is an advanced tool for genetic engineering and a useful model to investigate cut-and-paste DNA transposition in vertebrate cells. Here, we identify novel SB transposase mutants that display efficient and canonical excision but practically unmeasurable genomic re-...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Kesselring, Lisa, Miskey, Csaba, Zuliani, Cecilia, Querques, Irma, Kapitonov, Vladimir, Laukó, Andrea, Fehér, Anita, Palazzo, Antonio, Diem, Tanja, Lustig, Janna, Sebe, Attila, Wang, Yongming, Dinnyés, András, Izsvák, Zsuzsanna, Barabas, Orsolya, Ivics, Zoltán
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2020
Teme:
Molecular Biology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6943129/
https://ncbi.nlm.nih.gov/pubmed/31777924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1119
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