A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming

The Sleeping Beauty (SB) transposon is an advanced tool for genetic engineering and a useful model to investigate cut-and-paste DNA transposition in vertebrate cells. Here, we identify novel SB transposase mutants that display efficient and canonical excision but practically unmeasurable genomic re-...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Kesselring, Lisa, Miskey, Csaba, Zuliani, Cecilia, Querques, Irma, Kapitonov, Vladimir, Laukó, Andrea, Fehér, Anita, Palazzo, Antonio, Diem, Tanja, Lustig, Janna, Sebe, Attila, Wang, Yongming, Dinnyés, András, Izsvák, Zsuzsanna, Barabas, Orsolya, Ivics, Zoltán
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6943129/
https://ncbi.nlm.nih.gov/pubmed/31777924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz1119
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