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Methods for studying the radical SAM enzymes in diphthamide biosynthesis
Diphthamide is a unique posttranslational modification on translation elongation factor 2 (EF2) in archaea and eukaryotes. Biosynthesis of diphthamide was proposed to involve four steps. The first step is a C-C bond forming reaction catalyzed by unique radical S-adenosylmethionine (SAM) enzymes. Cla...
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| Publicado no: | Methods Enzymol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6927669/ https://ncbi.nlm.nih.gov/pubmed/30097101 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.04.001 |
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