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Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems

OBJECTIVE: Fluorescent labeling of specific cell-surface proteins enables a manifold of techniques to study their function in health and disease. A frequently cited family of methods employs phosphopantetheinyl transferases (PPTases) to attach probes, provided as conjugates of Coenzyme A. This metho...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:PLoS One
Prif Awduron: Stüber, Jakob C., Plückthun, Andreas
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2019
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6922365/
https://ncbi.nlm.nih.gov/pubmed/31856184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0226579
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