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Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling

Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PP...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Zhao, Bo, Zhang, Keya, Bhuripanyo, Karan, Wang, Yiyang, Zhou, Han, Zhang, Mengnan, Yin, Jun
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4648548/
https://ncbi.nlm.nih.gov/pubmed/25560074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2272-7_11
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