Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase

An 11-residue peptide with the sequence DSLEFIASKLA was identified from a genomic library of Bacillus subtilis by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by small molecule-CoA conjugates. We name this peptide the “ybbR tag,” because...

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Bibliographic Details
Main Authors: Yin, Jun, Straight, Paul D., McLoughlin, Shaun M., Zhou, Zhe, Lin, Alison J., Golan, David E., Kelleher, Neil L., Kolter, Roberto, Walsh, Christopher T.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2005
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1276090/
https://ncbi.nlm.nih.gov/pubmed/16236721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0507705102
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