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Measurement of Net Rate Constants from Enzyme Progress Curves without Curve Fitting
A method is described whereby net rate constants can be directly inferred from the progress curves of enzyme intermediates without the need for model specification, numerical analysis, curve fitting, or the steady-state approximation. Specifically, if an enzyme intermediate in an ultimately irrevers...
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| Published in: | Biochemistry |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6910778/ https://ncbi.nlm.nih.gov/pubmed/31710808 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.9b00762 |
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