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Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells

Mutations accumulating in hematopoietic stem and progenitor cells (HSPCs) during development can cause severe hematological disorders. Modeling these mutations in mice is essential for understanding their functional consequences. Here, we describe an efficient CRISPR/Cas9-based system to knock in an...

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Dades bibliogràfiques
Publicat a:Cell Rep
Autors principals: Tran, Ngoc Tung, Sommermann, Thomas, Graf, Robin, Trombke, Janine, Pempe, Jenniffer, Petsch, Kerstin, Kühn, Ralf, Rajewsky, Klaus, Chu, Van Trung
Format: Artigo
Idioma:Inglês
Publicat: Cell Press 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6899516/
https://ncbi.nlm.nih.gov/pubmed/31553918
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2019.08.065
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