Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells

Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse HSPCs....

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Tran, Ngoc Tung, Trombke, Janine, Rajewsky, Klaus, Chu, Van Trung
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7357675/
https://ncbi.nlm.nih.gov/pubmed/32685932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100028
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