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Unraveling Determinants of Affinity Enhancement in Dimeric Aptamers for a Dimeric Protein
High-affinity aptamers can be derived de novo by using stringent conditions in SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiments or can be engineered post SELEX via dimerization of selected aptamers. Using electrophoretic mobility shift assays, we studied a series of hete...
Enregistré dans:
| Publié dans: | Sci Rep |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group UK
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6883073/ https://ncbi.nlm.nih.gov/pubmed/31780794 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-54005-4 |
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