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Unraveling Determinants of Affinity Enhancement in Dimeric Aptamers for a Dimeric Protein

High-affinity aptamers can be derived de novo by using stringent conditions in SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiments or can be engineered post SELEX via dimerization of selected aptamers. Using electrophoretic mobility shift assays, we studied a series of hete...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Manochehry, Sepehr, McConnell, Erin M., Li, Yingfu
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6883073/
https://ncbi.nlm.nih.gov/pubmed/31780794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-54005-4
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