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Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale

Identification and characterization of small molecule–protein interactions is critical to understanding the mechanism of action of bioactive small molecules. Photo-affinity labeling (PAL) enables the capture of non-covalent interactions for enrichment and unbiased analysis by mass spectrometry (MS)....

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Publicat a:Curr Protoc Chem Biol
Autors principals: Flaxman, Hope A., Miyamoto, David K., Woo, Christina M.
Format: Artigo
Idioma:Inglês
Publicat: 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6880798/
https://ncbi.nlm.nih.gov/pubmed/31763793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.75
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