One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no ‘two-in-one’ KI strategy combining...

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Udgivet i:eLife
Main Authors: Li, Wenyuan, Zhang, Yage, Han, Bingzhou, Li, Lianyan, Li, Muhang, Lu, Xiaochan, Chen, Cheng, Lu, Mengjia, Zhang, Yujie, Jia, Xuefeng, Zhu, Zuoyan, Tong, Xiangjun, Zhang, Bo
Format: Artigo
Sprog:Inglês
Udgivet: eLife Sciences Publications, Ltd 2019
Fag:
Genetics and Genomics
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6845224/
https://ncbi.nlm.nih.gov/pubmed/31663848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.48081
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