One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no ‘two-in-one’ KI strategy combining...

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Опубликовано в: :eLife
Главные авторы: Li, Wenyuan, Zhang, Yage, Han, Bingzhou, Li, Lianyan, Li, Muhang, Lu, Xiaochan, Chen, Cheng, Lu, Mengjia, Zhang, Yujie, Jia, Xuefeng, Zhu, Zuoyan, Tong, Xiangjun, Zhang, Bo
Формат: Artigo
Язык:Inglês
Опубликовано: eLife Sciences Publications, Ltd 2019
Предметы:
Genetics and Genomics
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6845224/
https://ncbi.nlm.nih.gov/pubmed/31663848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.48081
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