One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no ‘two-in-one’ KI strategy combining...

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Dettagli Bibliografici
Pubblicato in:eLife
Autori principali: Li, Wenyuan, Zhang, Yage, Han, Bingzhou, Li, Lianyan, Li, Muhang, Lu, Xiaochan, Chen, Cheng, Lu, Mengjia, Zhang, Yujie, Jia, Xuefeng, Zhu, Zuoyan, Tong, Xiangjun, Zhang, Bo
Natura: Artigo
Lingua:Inglês
Pubblicazione: eLife Sciences Publications, Ltd 2019
Soggetti:
Genetics and Genomics
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6845224/
https://ncbi.nlm.nih.gov/pubmed/31663848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.48081
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