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Improved Cas9 activity by specific modifications of the tracrRNA
CRISPR/Cas is a transformative gene editing tool, that offers a simple and effective way to target a catalytic Cas9, the most widely used is derived from Streptococcus pyogenes (SpCas9), with a complementary small guide RNA (sgRNA) to inactivate endogenous genes resulting from insertions and deletio...
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| Published in: | Sci Rep |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Nature Publishing Group UK
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6834579/ https://ncbi.nlm.nih.gov/pubmed/31695072 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-52616-5 |
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