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Improved Cas9 activity by specific modifications of the tracrRNA

CRISPR/Cas is a transformative gene editing tool, that offers a simple and effective way to target a catalytic Cas9, the most widely used is derived from Streptococcus pyogenes (SpCas9), with a complementary small guide RNA (sgRNA) to inactivate endogenous genes resulting from insertions and deletio...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Scott, Tristan, Urak, Ryan, Soemardy, Citradewi, Morris, Kevin V.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6834579/
https://ncbi.nlm.nih.gov/pubmed/31695072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-52616-5
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