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crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus
The Cas9-crRNA complex of the Streptococcus thermophilus DGCC7710 CRISPR3-Cas system functions as an RNA-guided endonuclease with crRNA-directed target sequence recognition and protein-mediated DNA cleavage. We show here that an additional RNA molecule, tracrRNA (trans-activating CRISPR RNA), co-pur...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3737341/ https://ncbi.nlm.nih.gov/pubmed/23535272 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.24203 |
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