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A robust and economical pulse-chase protocol to measure the turnover of HaloTag fusion proteins
The self-labeling protein HaloTag has been used extensively to determine the localization and turnover of proteins of interest at the single-cell level. To this end, halogen-substituted alkanes attached to diverse fluorophores are commercially available that allow specific, irreversible labeling of...
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| Publicado no: | J Biol Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6827285/ https://ncbi.nlm.nih.gov/pubmed/31511325 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.010596 |
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