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Nano-mechanics of HaloTag Tethers

The active site of the Haloalkane Dehydrogenase (HaloTag) enzyme can be covalently attached to a chloroalkane ligand providing a mechanically strong tether, resistant to large pulling forces. Here we demonstrate the covalent tethering of protein L and I27 polyproteins between an AFM cantilever and a...

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Bibliografiske detaljer
Main Authors: Popa, Ionel, Berkovich, Ronen, Alegre-Cebollada, Jorge, Badilla, Carmen L., Rivas-Pardo, Jaime Andres, Taniguchi, Yukinori, Kawakami, Masaru, Fernandez, Julio M.
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3874216/
https://ncbi.nlm.nih.gov/pubmed/23909704
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja4056382
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