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Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism

Pichia pastoris KM71H (Mut(S)) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induction s...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Microb Biotechnol
Prif Awduron: Liu, Wan‐cang, Zhou, Fei, Xia, Di, Shiloach, Joseph
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: John Wiley and Sons Inc. 2019
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6801151/
https://ncbi.nlm.nih.gov/pubmed/31131547
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13420
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