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Expression of SARS-coronavirus spike glycoprotein in Pichia pastoris
To establish a rapid and economical method for the expression of viral proteins in high yield and purity by Pichia pastoris, the S protein of the SARS-CoV was selected in this study. Six S glycoprotein fragments were expressed in Escherichia coli BL21 and yeast KM71H strains. After purification by a...
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| 發表在: | Virus Genes |
|---|---|
| Main Authors: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Springer US
2008
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7088578/ https://ncbi.nlm.nih.gov/pubmed/18958613 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11262-008-0292-3 |
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