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Expression of SARS-coronavirus spike glycoprotein in Pichia pastoris

To establish a rapid and economical method for the expression of viral proteins in high yield and purity by Pichia pastoris, the S protein of the SARS-CoV was selected in this study. Six S glycoprotein fragments were expressed in Escherichia coli BL21 and yeast KM71H strains. After purification by a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Virus Genes
Hauptverfasser: Chuck, Chi-Pang, Wong, Chi-Hang, Chow, Larry Ming-Cheung, Fung, Kwok-Pui, Waye, Mary Miu-Yee, Tsui, Stephen Kwok-Wing
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer US 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7088578/
https://ncbi.nlm.nih.gov/pubmed/18958613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11262-008-0292-3
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