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Expression of SARS-coronavirus spike glycoprotein in Pichia pastoris

To establish a rapid and economical method for the expression of viral proteins in high yield and purity by Pichia pastoris, the S protein of the SARS-CoV was selected in this study. Six S glycoprotein fragments were expressed in Escherichia coli BL21 and yeast KM71H strains. After purification by a...

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Detalhes bibliográficos
Publicado no:Virus Genes
Main Authors: Chuck, Chi-Pang, Wong, Chi-Hang, Chow, Larry Ming-Cheung, Fung, Kwok-Pui, Waye, Mary Miu-Yee, Tsui, Stephen Kwok-Wing
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7088578/
https://ncbi.nlm.nih.gov/pubmed/18958613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11262-008-0292-3
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