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Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

The yeast Pichia pastoris is a common host for the recombinant production of biopharmaceuticals, capable of performing posttranslational modifications like glycosylation of secreted proteins. However, the activity of the OCH1 encoded α-1,6-mannosyltransferase triggers hypermannosylation of secreted...

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Bibliografische gegevens
Hoofdauteurs: Krainer, Florian W., Gmeiner, Christoph, Neutsch, Lukas, Windwarder, Markus, Pletzenauer, Robert, Herwig, Christoph, Altmann, Friedrich, Glieder, Anton, Spadiut, Oliver
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2013
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3834888/
https://ncbi.nlm.nih.gov/pubmed/24252857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03279
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