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Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism
Pichia pastoris KM71H (Mut(S)) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induction s...
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| Pubblicato in: | Microb Biotechnol |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
John Wiley and Sons Inc.
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6801151/ https://ncbi.nlm.nih.gov/pubmed/31131547 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13420 |
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