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A Single Reactive Noncanonical Amino Acid is Able to Dramatically Stabilize Protein Structure

A p-isothiocyanate phenylalanine mutant of the homodimeric protein homoserine o-succinyltransferase (MetA) was isolated in a temperature dependent selection from a library of metA mutants containing noncanonical amino acids. This mutant protein has a dramatic increase of 24°C in thermal stability co...

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Publicat a:ACS Chem Biol
Autors principals: Li, Jack C., Nastertorabi, Fariborz, Xuan, Weimin, Han, Gye Won, Stevens, Raymond C., Schultz, Peter G.
Format: Artigo
Idioma:Inglês
Publicat: 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6791372/
https://ncbi.nlm.nih.gov/pubmed/31181898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.9b00002
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